| Zhongliu Wu | |
| Title: | |
|---|---|
| Phone: | 028-82890423, 028-82890434 |
| Highest Education: | Ph.D. |
| Email: | wuzhl@cib.ac.cn |
Education and Appointments:
Address:
Chengdu Institute of Biology, Chinese Academy of Sciences
9 South Renmin Road, 4th Section, B.O.Box 416
Chengdu, 610041
China
Tel: 86-28-85238385
Fax: 86-28-85222753
E-mail: wuzhl@cib.ac.cn
Education:B. S. East China University of Science and Technology, Shanghai, P.R.China (Applied Organic Chemistry) 1998.
Ph.D. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, P.R.China (Organic Chemistry / Bioorganic Chemistry) 2003. (with professor Zu-Yi Li)
Professional Appointments:
2003.7-2003.9 Research Assistant, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China (with Professor Zu-Yi Li)
2007.5-present Professor, Center in Applied and Environmental Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China
Research Interest
1. Screening, molecular evolution & industrial application of oxidizing enzymes and reductases
2. Design and construction of new multi-enzyme biocatalytic systems and chemical-enzyme coupling systems
3. Diversity-oriented biotransformations for active compounds screening
4. Constructing a variety of active molecular libraries and novel drug-like molecules by biocatalysis
Public Services
Honors
2003 Yonglin Liu Scholarship for Outstanding Graduates, CAS
Seleted Publication
1. X.-Q. Pei; M.-Y. Xu; Wu, Z.-L.* Two “classical” Old Yellow Enzymes from Chryseobacterium sp. CA49: broad substrate specificity of 
Chr-OYE1 and limited activity of Chr-OYE2. J. Mol. Catal. B: Enzym. 2016, 123:91-99.
2. Zhao, F.-J.; Pei, X.-Q.; Ren, Z.-Q.; Wu, Z.-L.* Rapid asymmetric reduction of ethyl 4-chloro-3-oxobutanoate using a thermostabilized mutant of ketoreductase ChKRED20. Appl Microbiol Biotechnol. 2015. Doi：10.1007/s00253-015-7200-2
3. Li, J.-J.; Pei, X.-Q.; Zhang, S.-B.; Wu, Z.-L*. Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis. Process Biochemistry. 2015, 50: 1783-1787.
4. Li, J.-J.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L*. Removal of the free cysteine residue reduces irreversible thermal inactivation of Feruloyl Esterase: evidence from circular dichroism and fluorescence spectra. Acta Biochimica et Biophysica Sinica. 2015, 47(8):612-619. Doi：10.1093/abbs/gmv057
5. Liu, Y.-C; Wu, Z.-L*. Switchable asymmetric bio-epoxidation of a,b-unsaturated ketones. Chem. Commun. 2015, DOI: 10.1039/C5CC07548C
6. Ren, Z.-Q; Liu, Y.; Pei, X.-Q; Wang H.-B.; Wu, Z.-L*. Bioreductive production of enantiopure (S)-duloxetine intermediatescatalyzed with ketoreductase ChKRED15. J. Mol. Catal. B: Enzym. 2015, 113,76–81.
7. Liu, Y.-C; Liu, Y.; Wu, Z.-L*. Synthesis of enantiopure glycidol derivatives via a one-pot two-step enzymatic cascade. Org. Biomol. Chem., 2015, 13, 2146–2152
8. Xu, M.-Y., Pei, X.-Q., Wu, Z.-L.* Identification and characterization of a novel "thermophilic-like" Old Yellow Enzyme from the genome of Chryseobacterium sp. CA49. J. Mol. Catal. B: Enzym. 2014, 108, 64-71.
10. Liu, Y., Tang, T.-X., Pei, X.-Q., Zhang, C., Wu, Z.-L.* Identification of ketone reductase ChKRED20 from the genome of Chryseobacterium sp. CA49 for highly efficient anti-Prelog reduction of 3,5-bis(trifluoromethyl)acetophenone. J. Mol. Catal. B: Enz. 2014, 102(4), 1-8.
11. Wang, H.-B., Pei, X.-Q.; Wu, Z.-L.* An enoate reductase Acrh-OYE4 from Achromobacter sp. JA81: Characterization and application in asymmetric bioreduction of C=C bonds. Appl. Microbiol. Biotechnol. 2014, 98(2), 705-715.
12. Yi, Z.-L.; Zhang, S.-B.*; Pei, X.-Q.; Wu, Z.-L.* Design of mutants for enhanced thermostability of b-glycosidase BglY from Thermus thermophilus. Bioresour. Technol. 2013, 129, 629-633.
13. Lin, H.; Tang, D.-F.; Ahmed, A. A. Q.; Liu, Y.; Wu, Z.-L.* Mutations at the putative active cavity of styrene monooxygenase: enhanced activity and reversed enantioselectivity. J. Biotechnol. 2012, 161, 235-241.
14. Zhang, S.-B.; Pei, X.-Q.; Wu, Z.-L.* Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger. Bioresour. Technol. 2012, 117, 140-147.
15. Liu, Y.-J.; Pei, X.-Q.; Lin, H.; Gai, P.; Liu, Y.-C.; Wu, Z.-L.* Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase. Appl. Microbiol. Biotechnol. 2012, 95(3), 635-645.
16. Zhang, S.-B.; Ma, X.-F.; Pei, X.-Q.; Liu, J.-Y.; Shao, H.-W.*, Wu, Z.-L.* A practical high-throughput screening system for feruloyl esterases: substrate design and evaluation. J. Mol. Catal. B: Enzym. 2012, 74, 36-40.
17. Lin, H.; Liu, J.-Y.; Wang, H.-B.; Ahmed, A. A. Q.; Wu, Z.-L.* Biocatalysis as an alternative for the production of chiral epoxides: a comparative review. J. Mol. Catal. B: Enzym. 2011, 72(3-4), 77-89. (Invited review)
18. Tang, C.-G.; Lin, H.; Zhang, C.; Liu, Z.-Q.; Yang, T.; Wu, Z.-L.* Highly enantioselective bioreduction of N-methyl-3-oxo-3- (thiophen-2-yl) propanamide for the production of (S)-duloxetine. Biotechnol. Lett. 2011, 33(7), 1435-1440.
19. Lin, H.; Liu, Y.; Wu, Z.-L.* Highly diastereo- and enantio-selective epoxidation of secondary allylic alcohols catalyzed by styrene monooxygenase. Chem. Commun., 2011, 47, 2610-2612.
20. Lin, H.; Liu, Y.; Wu, Z.-L.* Asymmetric epoxidation of styrene derivatives by styrene monooxygenase from Pseudomonas sp. LQ26: effects of a- and b-substituents. Tetrahedron: Asymmetry, 2011, 22, 134-137.
21. Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresour. Technol. 2011, 102(3), 3636–3638
22. Pei, X.-Q.; Yi, Z.-L.; Tang, C.-G.; Wu, Z.-L.* Three amino acid changes contribute markedly to the thermostability of b-glucosidase BglC from Thermobifida fusca. Bioresour. Technol. 2011, 102(3), 3337–3342.
23. Qaed, A. A.; Lin, H.; Tang, D.-F.; Wu, Z.-L.* Rational design of styrene monooxygenase mutants with altered substrate preference. Biotechnol. Lett. 2011, 33, 611-616.
24. Zhang, S.-B.; Wu, Z.-L.* Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm. 
Bioresour. Technol. 2011, 102 (2), 2093–2096.
25. Lin, H.; Qiao, J.; Liu, Y.; Wu, Z.-L.* Styrene monooxygenase from Pseudomonas sp. LQ26 catalyzes the asymmetric epoxidation of both conjugated and unconjugated alkenes. J. Mol. Catal. B: Enzym. 2010, 67(3-4), 236-241.
26. Zhang, Z.-G.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis. Bioresour. Technol. 2010, 101(23), 9272-9278.
27. Yi, Z.-L.; Wu, Z.-L.* Mutations from a family-shuffling-library reveal amino acid residues responsible for the thermostability of endoglucanase CelA from Clostridium thermocellum Biotechnol. Lett. 2010, 32(12), 1869-1875.
28. Tang, Z., Salamanca-Pinzon, S.G., Wu, Z.L., Xiao, Y., Guengerich, F.P., Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function. Arch. Biochem. Biophys. 2010, 494(1), 86-93.
29. Wu, Z.-L.*; Qiao, J.; Zhang, Z.-G.; Guengerich, F.P.; Liu, Yan; Pei, X.-Q. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression. Biotechnol. Lett. 2009, 31(10), 1589-1593.
30. Zhang, Z.-G.; Liu, Y.; Guengerich, F.P.; Matse, J.H.; Chen J.; Wu, Z.-L.* Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries. J. Biotechnol. 2009, 139(1), 12-18.
31. Stark, K.; Wu, Z.-L.; Bartleson, C.J.; Guengerich, F.P. Heterologous expression and mRNA distribution of orphan cytochrome P450 20A1. Drug Metab. Dispos. 2008, 36(9), 1930-1937.
32. Wu, Z.-L.; Sohl, C.D.; Shimada, T.; Guengerich, F.P. Recombinant Enzymes Over expressed in Bacteria Show Broad Catalytic Specificity of Human Cytochrome P450 2W1 and Limited Activity of Human Cytochrome P450 2S1. Mol. Pharmacol., 2006, 69(6), 2007-2014.
33. Wu, Z.-L.; Bartleson, C.J.; Ham, A.J.; Guengerich, F.P. Heterologous Expression, Purification, and Properties of human cytochrome P450 27C1, Arch. Biochem. Biophys. 2006, 445(1), 138-146.
34. Wu, Z.-L.; Podust, L.M.; Guengerich, F.P. Expansion of Substrate Specificity of Cytochrome P450 2A6 by Random and Site-Directed Mutagenesis. J. Biol. Chem. 2005, 280(49), 41090-41100.
35. Kim, D.; Wu, Z.-L.; Guengerich, F.P. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem. 2005, 280(48), 40319-40327.
36. Wu, Z.-L.; Aryal, P.; Lozach, O.; Meijer, L.; Guengerich, F.P. Biosynthesis of New Indigoid Inhibitors of Protein Kinases Using Recombinant Cytochrome P450 2A6, Chem. Biodiv. 2005, 2(1), 51-65.
37. Guengerich, F.P.; Wu, Z.-L.; Bartleson, C.J. Function of Human Cytochrome P450s: Characterization of the Orphans. Biochem. Biophys. Res. Commun. 2005, 338(1), 465-469.
38. Wu, Z.-L.; Li, Z.-Y. Highly Enantioselective Synthesis of a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Dinitriles with Rhodococcus sp. CGMCC 0497, Chem. Commun. 2003, 3, 386-387. (Selected by ChemInform, 2003, 34(23), Nitriles Q0520.)
39. Wu, Z.-L.; Li, Z.-Y. Practical Synthesis of Optically Active a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Malonamide Derivatives with Rhodococcus sp. CGMCC 0497, J. Org. Chem. 2003, 68(6), 2479-2482.
40. Wu, Z.-L.; Li, Z.-Y. Biocatalytic Asymmetric Hydrolysis of (±)-b-Hydroxy Nitriles by Rhodococcus sp. CGMCC 0497, J. Mol. Catal. B: Enzym. 2003, 22(1-2), 105-112.
41. Wu, Z.-L.; Li, Z.-Y. Enantioselective Biotransformation of a,a-Disubstituted Dinitriles to the Corresponding 2-Cyano-Acetamides using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry, 2003, 14(15), 2133-2142.
42. Wu, Z.-L.; Li, Z.-Y. Enhancement of Enzyme Activity and Enantioselectivity via Cultivation in Nitrile Metabolism by Rhodococcus sp. CGMCC 0497, Biotechnol. Appl. Biochem. 2002, 35(1), 61-67.
43. Wu, Z.-L.; Li, Z.-Y. Enantioselective Hydrolysis of Various Racemic a-Substituted Arylacetonitriles using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry 2001, 12(23), 3305-3312.
44. Li, Z.-Y., Wu, Z.-L., Chen, Y. (李祖义，吴中柳，陈颖) Evolution of Industrialization of Biocatalysis (生物催化产业化进展), Chin. J. Org. Chem. (有机化学) 2003, 23(12), 1446-1451.
45. Li, Z.-Y., Wu, Z.-L., Li, J. (李祖义，吴中柳，励俊) New Methods in Immobilization: Biocapsulation by Epoxy-amine Resins and Silicone Elastomers (新型固定化方法－环氧胺树脂及硅树脂生物包埋) Chin. J. Org. Chem. (有机化学) 2003, 23, 150-154.
46. Wu, Z.-L.; Li, Z.-Y. (吴中柳，李祖义) Microbial and Enzymatic Hydrolysis of Nitriles in Organic Synthesis (氰基水解酶在有机合成中的应用), Chin. J. Org. Chem. (有机化学) 2001, 21, 25-32.
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